The Football Association, FA, has confirmed that Kyle Walker and Harry Maguire have left the England camp with injuries.
The duo, along with goalkeeper Sam Johnstone, will return to their respective clubs for further assessment.
Walker was substituted in the 20th minute against Brazil on Saturday night, after appearing to injure his hamstring.
Maguire lasted 67 minutes in the same game, before being replaced by Lewis Dunk.
These withdrawals are a fresh blow to England manager, Gareth Southgate, who has already seen Harry Kane and Bukayo Saka pull out of the squad.
Kane, who was in the stands during England’s loss to Brazil on Saturday, had linked up with the squad ahead of the game despite picking up an ankle injury in Bayern Munich’s win over Darmstadt before the international break.